Anti-mycobacterial and immunomodulatory activity of n-hexane fraction and spathulenol from Ocotea notata leaves

Abstract Ocotea notata (Lauraceae) is popularly known as white-cinnamon. species have several medicinal uses, especially for treating chest pain, rheumatism and wounds. The present study aimed to analyze the chemical composition of O. n-hexane fraction, in addition its anti-mycobacterial immunomodulatory activities. fraction was analyzed by GC-MS chromatographed afford 15 subfractions (SF1-15), where SF5 identified, NMR, sesquiterpene spathulenol. most potent inhibiting nitric oxide (NO) tumor necrosis factor-alpha (TNF-α) production on LPS-stimulated macrophages (IC50 8.3 ± 0.9 5.9 ±1.0 μg/mL, respectively). SF4, a major subfraction, that presents spathulenol analogous constituent, also inhibited NO TNF-α production. Spathulenol only modulated 45.6 1.4 μg/mL). revealed antimycobacterial activity against Mycobacterium bovis BCG, M. tuberculosis H37Rv, M299 strains. growth Mtb H37Rv with MIC50 36.9 1.5 μg/mL (167.5 6.8 μM), MIC5042.1 0.5 (191.0 2.2 μM). This first report describing isolation from leaves activity.